Contact an Account ManagerUltra-high performance liquid chromatography coupled to high resolution Orbitrap mass spectrometry for metabolomic profiling of the endogenous phytohormonal status of the tomato plant
Summary
Type: HPLC
Application #: 126130
Detection:
Conditions: Eluent A: 0.1 % formic acid in ultrapure water
Eluent B: methanol
Gradient: 98 % A for 1.00 min, 98-60 % A in 1.50 min, 60-50 % A in 1.50 min, 50-20 % A in 1.00 min, 20 % A for 2.00 min, 20-0 % A in 0.10 min, 0 % A for 0.90 min, 0-98 % in 0.01 min, re-equilibration for 2.00 min, divert valve for 1.60 min (to avoid caramel formation)
Flow rate: 300 µL/min
Temperature: 30 °C
Injection volume: 10 µL
Authors: Van Meulebroek, L. et al.
Source: J. Chromatogr. A 1260 (2012) 67–80
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